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A Basic Guide to Real Time PCR in Microbial Diagnostics: Definitions, Parameters, and Everything

real-time qPCR

Real time PCR (quantitative PCR, qPCR) is now a well-established method for the detection, quantification, and typing of different microbial agents in the areas of clinical and veterinary diagnostics and food safety. Although the concept of PCR is relatively simple, there are specific issues in qPCR that developers and users of this technology must bear in mind. These include the use of correct terminology and definitions, understanding of the principle of PCR, difficulties with interpretation and presentation of data, the limitations of qPCR in different areas of microbial diagnostics and parameters important for the description of qPCR performance. It is not our intention in this review to describe every single aspect of qPCR design, optimization, and validation; however, it is our hope that this basic guide will help to orient beginners and users of qPCR in the use of this powerful technique. Continue reading A Basic Guide to Real Time PCR in Microbial Diagnostics: Definitions, Parameters, and Everything

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African Swine Fever RT-PCR Kit validated by Ministry of Agriculture

According to the announcement from Ministry of Agriculture and Rural Affairs, RINGBIO ASFV RT-PCR Kit is validated against OIE Asf protocols. The results showed that RINGBIO ASFV RT-PCR kit is working properly in terms of the sensitivity, specificity and reproducibility. The operation is simple and easy, which are then recommended national wide to detect ASF. Continue reading African Swine Fever RT-PCR Kit validated by Ministry of Agriculture