Establishing a sandwich ELISA for the detection of african swine fever virus
African swine fever virus is a large, double-stranded DNA virus in the Asfarviridae family. It is the causative agent of African swine fever. The virus causes a haemorrhagic fever with high mortality rates in domestic pigs; some isolates can cause death of animals as quickly as a week after infection. As it is spreading quickly across Asia, it causes severe damage to swine industry.
To emliminate ASF from the herd, the first step is identify it. The normal most used method is RT-PCR, which is to detect ASF DNA and its fraction in various biological samples. Blood, tissue, spleen, environmental samples can be tested. However, sometimes it is difficult to set a molecular lab in farm, it becomes necessary to test with immunoassays, such as ELISA, lateral flow immunochromatography, etc.
To detect ASF virus by immunoassay, P72, P30, etc are frequently employed as the target protein, thus specific anitbodies against which are then used to establish assays. Due to the complicated structure of these protein, it is difficult to prepare the active recombinant full-length protein. Researchers tends to use commercial antibodies to start the assay. Here at Antibodymall.com, we have these antibodies pair readily available for your experiment, they are:
- Anti African swine fever virus monoclonal antibody / clone #1 AB3009
- Anti African swine fever virus monoclonal antibody / clone #2 AB3010
These two antibodies are highly specific to ASF p72 protein, and are already validated in commercial ELISA kit and rapid test kit.
If you are about to establish you own assays, these are your choices. If you are just trying some tests, you can also find the below test kits.
- African Swine Fever Virus antigen ASFV Ag ELISA Kit E39021
- African Swine Fever Virus antibody ASFV Ab ELISA Kit E39011
- Sastre P, Gallardo C, Monedero A, et al. Development of a novel lateral flow assay for detection of African swine fever in blood. BMC Vet Res. 2016;12:206. Published 2016 Sep 15. doi:10.1186/s12917-016-0831-4
- Geoffrey H.Hutchings, Nigel P.Ferris, Indirect sandwich ELISA for antigen detection of African swine fever virus: Comparison of polyclonal and monoclonal antibodies, Journal of Virological Methods, Volume 131, Issue 2, February 2006, Pages 213-217, https://doi.org/10.1016/j.jviromet.2005.08.009