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A Basic Guide to Real Time PCR in Microbial Diagnostics: Definitions, Parameters, and Everything

real-time qPCR

Real time PCR (quantitative PCR, qPCR) is now a well-established method for the detection, quantification, and typing of different microbial agents in the areas of clinical and veterinary diagnostics and food safety. Although the concept of PCR is relatively simple, there are specific issues in qPCR that developers and users of this technology must bear in mind. These include the use of correct terminology and definitions, understanding of the principle of PCR, difficulties with interpretation and presentation of data, the limitations of qPCR in different areas of microbial diagnostics and parameters important for the description of qPCR performance. It is not our intention in this review to describe every single aspect of qPCR design, optimization, and validation; however, it is our hope that this basic guide will help to orient beginners and users of qPCR in the use of this powerful technique. Continue reading A Basic Guide to Real Time PCR in Microbial Diagnostics: Definitions, Parameters, and Everything

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Establishing a sandwich ELISA for the detection of african swine fever virus

African swine fever virus is a large, double-stranded DNA virus in the Asfarviridae family. It is the causative agent of African swine fever. The virus causes a haemorrhagic fever with high mortality rates in domestic pigs; some isolates can cause death of animals as quickly as a week after infection. As it is spreading quickly across Asia, it causes severe damage to swine industry.
Continue reading Establishing a sandwich ELISA for the detection of african swine fever virus

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What do you need for a rapid test laboratory?

What do you need for a rapid test laboratory? Rapid tests are designed for use where a preliminary screening test result is required and are especially useful in resource-limited settings. Actually for most of rapid test kits, you will need nothing extra. Just the test kit itself is enough. Continue reading What do you need for a rapid test laboratory?

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African Swine Fever RT-PCR Kit validated by Ministry of Agriculture

According to the announcement from Ministry of Agriculture and Rural Affairs, RINGBIO ASFV RT-PCR Kit is validated against OIE Asf protocols. The results showed that RINGBIO ASFV RT-PCR kit is working properly in terms of the sensitivity, specificity and reproducibility. The operation is simple and easy, which are then recommended national wide to detect ASF. Continue reading African Swine Fever RT-PCR Kit validated by Ministry of Agriculture

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African Swine Fever Rapid Test kits

African swine fever is a highly contagious and deadly viral disease affecting both domestic and wild pigs of all ages. ASF is not a threat to human health and cannot be transmitted from pigs to humans. It is not a food safety issue. As a key to ASFV eradication program, antibody testing shows great significance and importance in the very beginning and in the whole process. To detect the antibody in swine serum and plasma samples, high sensitivity, reproducibility and stability are required. Continue reading African Swine Fever Rapid Test kits