Real time PCR (quantitative PCR, qPCR) is now a well-established method for the detection, quantification, and typing of different microbial agents in the areas of clinical and veterinary diagnostics and food safety. Although the concept of PCR is relatively simple, there are specific issues in qPCR that developers and users of this technology must bear in mind. These include the use of correct terminology and definitions, understanding of the principle of PCR, difficulties with interpretation and presentation of data, the limitations of qPCR in different areas of microbial diagnostics and parameters important for the description of qPCR performance. It is not our intention in this review to describe every single aspect of qPCR design, optimization, and validation; however, it is our hope that this basic guide will help to orient beginners and users of qPCR in the use of this powerful technique. Continue reading A Basic Guide to Real Time PCR in Microbial Diagnostics: Definitions, Parameters, and Everything
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- Antibodies for antibiotic residues: colistin (polymyxin E), spiramycin, etc.
HAMBURG, July 16 (Reuters) – The first cases of African swine fever have been confirmed in farm pigs in Germany, the country’s federal agriculture ministry said on Friday.
The disease was confirmed in pigs on two farms in the eastern German state of Brandenburg.
Continue reading Germany has first African swine fever case in farm pigs
African swine fever virus is a large, double-stranded DNA virus in the Asfarviridae family. It is the causative agent of African swine fever. The virus causes a haemorrhagic fever with high mortality rates in domestic pigs; some isolates can cause death of animals as quickly as a week after infection. As it is spreading quickly across Asia, it causes severe damage to swine industry.
Continue reading Establishing a sandwich ELISA for the detection of african swine fever virus
What do you need for a rapid test laboratory? Rapid tests are designed for use where a preliminary screening test result is required and are especially useful in resource-limited settings. Actually for most of rapid test kits, you will need nothing extra. Just the test kit itself is enough. Continue reading What do you need for a rapid test laboratory?
According to the announcement from Ministry of Agriculture and Rural Affairs, RINGBIO ASFV RT-PCR Kit is validated against OIE Asf protocols. The results showed that RINGBIO ASFV RT-PCR kit is working properly in terms of the sensitivity, specificity and reproducibility. The operation is simple and easy, which are then recommended national wide to detect ASF. Continue reading African Swine Fever RT-PCR Kit validated by Ministry of Agriculture
African swine fever is a highly contagious and deadly viral disease affecting both domestic and wild pigs of all ages. ASF is not a threat to human health and cannot be transmitted from pigs to humans. It is not a food safety issue. As a key to ASFV eradication program, antibody testing shows great significance and importance in the very beginning and in the whole process. To detect the antibody in swine serum and plasma samples, high sensitivity, reproducibility and stability are required. Continue reading African Swine Fever Rapid Test kits
The deadly pig virus that jumped from Africa to Europe is now ravaging China’s $128 billion pork industry and spreading to other Asian countries, an unprecedented disaster that has prompted Beijing to slaughter millions of pigs. But stopping African swine fever isn’t so easy. Continue reading How African Swine Fever is changing the swine industry in China?
Fur, fine, soft, hairy covering or coat of mammals that has been important to humankind throughout history, chiefly for warmth but also for decorative and other purposes. Continue reading Fur animals at a glance
Foot-and-mouth disease or hoof-and-mouth disease is an infectious and sometimes fatal viral disease that affects cloven-hoofed animals, including domestic and wild bovids. The virus causes a high fever for between two and six days, followed by blisters inside the mouth and on the feet that may rupture and cause lameness. Continue reading Swine foot and mouth disease